Dr. Paresh Jain

Affiliation : Associate Director, Clinical Solutions Platform, BD Biosciences Greater Asia, Singapore

Title of the Talk/Lab :Multicolor Panel design

Dr Paresh Jain is a hemat-oncopathologist currently working as Associate Director, Clinical Solutions Platform, BD Biosciences Greater Asia. Dr.  Jain qualified as M.D. (Pathology) from Grant Medical College, University of Bombay, India and trained in hemato-oncopathology at the All India Institute for Medical Sciences (AIIMS), New Delhi and at The Royal Marsden NHS Trust Hospital, London. He worked as Assistant Professor at AIIMS, New Delhi (2001-2005). In 2005, he joined BD Biosciences, India as Scientific Advisor where he has played an active role in institutionalizing CD4 GLP training programs for NACO sites, giving shape and direction to structured training courses in flow cytometry (where more than 1000 scholars have received hands-on training on flow cytometry) and establishing several BD Centers of Excellence for education and training in flow cytometry at Bangalore (BD-NCBS CoE), Kolkata (BD-CU CoE), Lucknow (BD-CSIR CDRI CoE), Pune (BD-NCCS CoE), Mumbai (BD-Hinduja Hospital CoE) and New Delhi (BD-JH FACSAcademy). Dr. Paresh has more than 35 national/international publications and has organized and/or joined as faculty in more than 100 CMEs/workshops/ training programs on clinical and research applications of flow cytometry. He is an active member of the ISAC’s Live Education Task Force and a founder member of The Cytometry Society, India

Lecture/Lab: Multicolor Panel design: Looking for the expression of specific cellular proteins is the most common application of flow cytometry but with the proliferation of fluorochromes, designing a multicolor panel can be challenging. In this lab, he will cover grammar and vocabulary of fluorochrome characteristics that are important from a panel design perspective with a focus on impact of spectral spillover related spread in limiting the ability to resolve weakly expressed antigens.

Relevant Literature:

1. Maciorowski, Z, Chattopadhyay, P.K. and Jain, P. Basic multicolor flow cytometry. Current Protocols in Immunology, 2017.117, 5.4.1-5.4.38.
2. Nguyen R, Perfetto S, Mahnke YD, Chattopadhyay P, Roederer M.Quantifying spillover spreading for comparing instrument performance and aiding in multicolor panel design. Cytometry A. 2013 Mar;83(3):306-15.

3. Maecker H and Trotter J. Selecting Reagent for Multicolor Applications. Application Note (June 2012): BD Biosciences, San Jose.
4. Alan Stall. Qr and Br in BD FACSDiva™ Software: Parameters for Characterizing Detector Performance. Application Note (June 2012) : BD Biosciences, San Jose