Affiliation : Director of the Flow Cytometry Core Technologies in the University College Dublin
Title of the Talk/Lab :Know Your Cytometer (KYC) & Setup of the Instrument
Alfonso Blanco is an Associate Professor and the Director of the Flow Cytometry Core Technologies in the University College Dublin. He is also the European distributor and Instructor of Expert Cytometry (ExCyte), Co-Director and founder of the Cytometry Society of Ireland, past-Councilor of International Society for the Advancement of Cytometry (ISAC), President and co-founder of the Spanish Research Society in Ireland (SRSI), co-founder and member of the Red de Asociaciones de Científicose Investigadores Españolesenel Extranjero (RAICEx),co-founder and coordinator of the Red de Iberoamericana de Citometríaand Scientific Advisor of the companies Sothic BioScience, Radisens Diagnostics and Java Clinical Research. Alfonso Blanco has been involved in the beta-testing of software, reagents and instruments from many of the major manufacturers in cytometry and is acting as one of the Beckman Coulter reference labs.
Alfonso organizes the UCD Winter and Summer School, as well as the ExCyte courses in Europe. He is the co-organiser of several national and international courses, workshops and conferences. Alfonso was involved in the development of CYTO University material and acts as chair of Instrument 4 Science, and as a member of ISAC Associated Societies and ISAC Membership Services Committee, ISAC Fundraising Task Force,CYTO2021 Meeting Committee as well as member of the European Society of Clinical Cytometry Analysis Website & Communication Committee. He is member of the IgR Committee (Gender Equality Committee of RAICEX).Alfonso is acting as administrator of ISAC, CSI, Red IberoamericanaCitometríaand SRSI Facebook sites.
Alfonso won the Accuri´s Creativity Awards 2009 “Innovative Applications of Flow Cytometry”, the Irish Laboratory Awards 2013 “Laboratory Staff Member of the Year” and was finalist of the Irish Laboratory Awards 2013 “Educational Laboratory of the Year”. Together with Sothic BioScience have reached the finals of the “Irish Times Innovation Awards2016” and with his support Sothic Bioscience competed in the “BOI Startup Awards (shortlisted 2017)” and won First Prize in the “Ireland Funds Business Plan Competition 2016”. Alfonso Blanco has been selected by ISAC as 1 of the 5 ISAC Scholars 2010-2015.
Lecture: Know Your Cytometer (KYC) & Setup of the Instrument: There are many aspects in a flow cytometry which will make us have a successful/unsuccessful assay. One of them is the sample preparation, as described in the other talk, but the other one is to get access to the right instrument and optimise it to get the maximum amount of information minimising the amount of potential errors and mistakes. During this presentation, we will provide different tips and tricks to optimise the instrument from maintenance and cleaning, to optimise the labelling method and detectors, how to compensate (if necessary), etc. Many of these aspects will require controls, controls and controls, and we will also describe what, when and why these controls are needed and how to use them. Different references will be included in the middle of the presentation, to make it easier to identify topics with literature cited.
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January 25th, 2022
