Dr. Paresh Jain

Affiliation : Associate Director, Clinical Solutions Platform, BD Biosciences, Central and South Asia and Japan

Title of the Talk/Lab :Analysis of Sepsis using Flow Cytometry

Dr Paresh Jain is a hemat-oncopathologist currently working as Associate Director, Clinical Solutions Platform, BD Biosciences Central and South Asia and Japan. Dr Jain qualified as M.D. (Pathology) from Grant Medical College, University of Bombay, India and trained in hemato-oncopathology at the All India Institute for Medical Sciences (AIIMS), New Delhi and at The Royal Marsden NHS Trust Hospital, London. He worked as Assistant Professor at AIIMS, New Delhi (2001-2005). In 2005, he joined BD Biosciences, India as Scientific Advisor where he played an active role in institutionalizing CD4 GLP training programs for NACO sites, giving shape and direction to structured training courses in flow cytometry (where more than 1000 scholars have received hands-on training on flow cytometry) and establishing several BD Centers of Excellence for education and training in flow cytometry at Bangalore (BD-NCBS CoE), Kolkata (BD-CU CoE), Lucknow (BD-CSIR CDRI CoE), Pune (BD-NCCS CoE), Mumbai (BD-Hinduja Hospital CoE) and New Delhi (BD-JH FACSAcademy). Dr Paresh has more than 40 national/international publications and has organized and/or joined as faculty in about 150 CMEs/workshops/ training programs on clinical and research applications of flow cytometry. He is an active member of the ISAC’s Live Education Task Force and a founder member of The Cytometry Society, India

Lecture: Analysis of Sepsis using Flow Cytometry:

Flow cytometry is a technology that allows quantitative measurements of multiple cellular characteristics at a single cell level. Expression of cellular antigens, detected by fluorochrome-conjugated antibodies, can be reported either as its ‘frequency’ (percent positive) in a gated population or as its fluorescence ‘intensity’ on a relative scale. A standardized quantitative methodology for defining intensity of marker expression is important particularly when characterizing antigens with heterogeneous expression or when their expression is altered in a diseased state.

Over the last decade various flow cytometry assays have been explored to identify activation of cells of innate immunity as markers of sepsis that detect infection earlier than the acute phase reactants. Up-regulated expression of CD64, the Fc-gamma receptor1, and CD11b (Integrin alpha M) on neutrophils and down-regulated expression of HLA-DR (MHC class II cell surface receptor involved in antigen presentation to T cells) on monocytes have shown promise in various studies for diagnosis, prognostication or monitoring response to antibiotics in a clinical setting of sepsis.

In this lecture/tutorial, he will present an approach to fluorescence quantitation of ‘cell bound’ antigens by flow cytometry using calibrated standardized PE-conjugated beads (BD Quantibrite), describe a method for fluorescence quantitation of CD64 on neutrophils and HLA-DR on monocytes, and share examples of deploying this assay in a clinical setting of sepsis.

Relevant Literature: