Dr. Paul Hutchinson

Affiliation : National University of Singapore

Title of the Talk/Lab :Sample Preparation for Flow Cytometry Experiment

Paul Hutchinson began working in flow cytometry in 1983 at the Peter MacCallumCancer Institute in Melbourne while completinghisApplied Physics degree at RMIT. In this lab, flow cytometry was used for clinical and research work, including the diagnosis of AIDS patients at the beginning of the HIV epidemic and early research on the isolation of hemopoietic stem cells. In 1986 he moved to Prince Henry’s Hospital to be in charge of the Cell Sorter in the Department of Nephrology. During his time in this lab, he completed his Master of Science degree (Monash University) on macrophage function. In 1992 Paul joined the Clinical Immunology department at Monash Medical Centre and was in charge of the core flow cytometry facility, which had 3 Mo-Flo cell sorters along with one analyzer. The lab was used for clinical tests and research, and it was here that he did his Ph.D. (Monash University) on the use of flow cytometry to quantify the level of immune function in renal transplant recipients. In February 2008 he was appointed the head of the Unidade de Citometria de Fluxo at the Instituo de Medicina Molecular in Lisbon, Portugal. This flow cytometry lab serviced more than 250 researchers and had a 11-color FACSAria cell sorter and three analyzers. Since July 2009 Paul has been in charge of the core flow cytometry facility at the Immunology Programme of the National University of Singapore which has4 analyzers (5 laser Aurora spectral analyzer, 5 laser X-20, 4 laser Fortessa, and 4 laser Attune), plus a Mo-Flo XDP cell sorter, Sony Sy3200 sorter, and a 5 laser 20 parameter BD FACSFusion cell sorter. Besides running this busy lab, Paul has pursued his research interests in using flow cytometry to develop diagnostic tests for tuberculosis and investigating new technologies for doing single-cell measurements.

Lecture: Sample Preparation for Flow Cytometry Experiment : In this lecture Paul will discuss methods for preparing samples for flow cytometry measurement. The focus will be on the techniques for doing immunofluorescent labelling of cells, including whole blood staining.

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